Purebacterialcolonies

Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following:

 

Exercise4: Purebacterialcolonies

 

1.   Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?

 

2.   Distinguishbetweena purecultureandamixedculture.

 

3.   Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished.

 

4.   Whyshoulda Petridishnotbe leftopenforanyextendedperiod?

 

5.   Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies?

 

 

Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures

 

1.   Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinical specimens.

 

2.   Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora?

 

3.   Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens?

 

4.   Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures?

 

5.   Whenmorethanonecolonytypeappearsinpureculture,whatarethemostlikelysourcesof extraneouscontamination?

 

 

Exercise3: Primarymediaforisolationofmicroorganisms

 

1.Definea differentialmediumanddiscussitspurpose.

 

2.Definea selectivemediumanddescribeitsuses.

 

3.WhyisMacConkeyagarselectiveas wellasdifferential?

 

4.Whyisbloodagarusefulasaprimaryisolationmedium?

 

 

5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar?

 

Support your responses with examples.

 

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